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Hsp70 antibody [001]

Anti-Hsp70 antibody [001] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01985
Anti-Hsp70 antibody [001] used in Flow cytometry (FACS). GTX01985
Anti-Hsp70 antibody [001] used in IHC (Paraffin sections) (IHC-P). GTX01985
Anti-Hsp70 antibody [001] used in IHC (Paraffin sections) (IHC-P). GTX01985
Anti-Hsp70 antibody [001] used in Western Blot (WB). GTX01985
Anti-Hsp70 antibody [001] used in Immunoprecipitation (IP). GTX01985

Cat No. GTX01985

Host

Rabbit

Clonality

Monoclonal

Clone Name

001

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Cynomolgus monkey
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:20-1:100
IHC-P 1:500-1:2000
FACS 1:25-1:100
IP 1-4 μl/mg of lysate
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human HSP70 / HSPA1A protein

Purification

Purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm , Nucleus

Database

DATA IMAGES

Anti-Hsp70 antibody [001] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01985

GTX01985 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01985 Hsp70 antibody [001].
Red : Primary antibody
Blue : DAPI
Dilution : 1:60
Permeabilzation : 0.3% Triton X-100

Anti-Hsp70 antibody [001] used in Flow cytometry (FACS). GTX01985

GTX01985 FACS Image

FACS analysis of HeLa cells using GTX01985 Hsp70 antibody [001].
The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-Hsp70 antibody [001] used in IHC (Paraffin sections) (IHC-P). GTX01985

GTX01985 IHC-P Image

IHC-P analysis of human hepatoma tissue section using GTX01985 Hsp70 antibody [001].
Dilution : 1:1000

Anti-Hsp70 antibody [001] used in IHC (Paraffin sections) (IHC-P). GTX01985

GTX01985 IHC-P Image

IHC-P analysis of cynomolgus macaque testis tissue section using GTX01985 Hsp70 antibody [001].
Dilution : 1:1000

Anti-Hsp70 antibody [001] used in Western Blot (WB). GTX01985

GTX01985 WB Image

WB analysis of various samples using GTX01985 Hsp70 antibody [001].
Lane A : A549 whole cell lysate
Lane B : HCT116 whole cell lysate
Lane C : HeLa whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-Hsp70 antibody [001] used in Immunoprecipitation (IP). GTX01985

GTX01985 IP Image

IP analysis of HeLa whole cell lysate using GTX01985 Hsp70 antibody [001].
IP antibody : 2 μl per 0.5 mg total whole cell lysate
Dilution : 1:200
50 % Protein G agarose : 15 μl

REFERENCE

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REVIEW

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Package List Price ($)
$ 339