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Hsp70 antibody [2A4]

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442
Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442
Anti-Hsp70 antibody [2A4] used in Western Blot (WB). GTX25442
Anti-Hsp70 antibody [2A4] used in Western Blot (WB). GTX25442
Anti-Hsp70 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX25442
Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442
Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442
Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442
Anti-Hsp70 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX25442
Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442
Anti-Hsp70 antibody [2A4] used in Immunoprecipitation (IP). GTX25442
Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442
Anti-Hsp70 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX25442

Cat No. GTX25442

Host

Mouse

Clonality

Monoclonal

Clone Name

2A4

Isotype

IgM

Application

WB, ICC/IF, IHC-P, IP, Neutralizing/Inhibition

Reactivity

Human, Mouse, Rat, Zebrafish, Yeast, Drosophila, Amphibians, Fish, Primate, Avian
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:2500
ICC/IF 1:100-1:200
IHC-P 1:200
IP 2 μg
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Product Note

This antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 437-479 of human HSP70.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

GTX25442 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25442 Hsp70 antibody [2A4].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

GTX25442 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

Anti-Hsp70 antibody [2A4] used in Western Blot (WB). GTX25442

GTX25442 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4].
Dilution : 1:1000

Anti-Hsp70 antibody [2A4] used in Western Blot (WB). GTX25442

GTX25442 WB Image

WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4].
Dilution : 1:1000

Anti-Hsp70 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX25442

GTX25442 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

GTX25442 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight in a humidified chamber

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

GTX25442 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

GTX25442 ICC/IF Image

ICC/IF analysis of NCI-H1299 Cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp70 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX25442

GTX25442 IHC-P Image

IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

GTX25442 ICC/IF Image

ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight in a humidified chamber

Anti-Hsp70 antibody [2A4] used in Immunoprecipitation (IP). GTX25442

GTX25442 IP Image

IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

GTX25442 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp70 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX25442

GTX25442 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf