Hsp70 antibody [2A4]

Name: Hsp70 antibody [2A4]
Brand: GeneTex
SKU: GTX25442
Price: 399.00 USD

Anti-Hsp70 antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX25442

Anti-Hsp70 antibody [2A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25442

Anti-Hsp70 antibody [2A4] used in Immunoprecipitation (IP). GTX25442

Anti-Hsp70 antibody [2A4] used in Western Blot (WB). GTX25442

Cat. No. GTX25442

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	2A4
Isotype	IgM
Application	WB, ICC/IF, IHC-P, IP, Neutralizing/Inhibition
Reactivity	Human, Mouse, Rat, Zebrafish, Yeast, Drosophila, Amphibians, Fish, Primate, Avian
Package	100 μl ($399)

See all Hsp70 products

View product citations for antibody GTX25442 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000 - 1:2500
ICC/IF	1:100-1:200
IHC-P	1:200
IP	2 μg
Neutralizing/Inhibition	Assay dependent

Not tested in other applications.

Product Note

This antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 437-479 of human HSP70.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Gene ID: 3303 HSPA1A
Gene ID: 3304 HSPA1B
UniProt: P0DMV9 HSPA1A
UniProt: P0DMV8 HSPA1B

Research Area

DATA IMAGES

GTX25442 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX25442 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

GTX25442 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

GTX25442 IP Image

IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

GTX25442 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25442 Hsp70 antibody [2A4].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

GTX25442 IHC-P Image

IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX25442 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight in a humidified chamber

GTX25442 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4].
Dilution : 1:1000

GTX25442 WB Image

WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4].
Dilution : 1:1000

GTX25442 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX25442 ICC/IF Image

ICC/IF analysis of NCI-H1299 Cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

GTX25442 ICC/IF Image

ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight in a humidified chamber

GTX25442 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

REFERENCE

Application Reference

Moore NS et al. Cells 2021; 10 (3) Critical Effects on Akt Signaling in Adult Zebrafish Brain Following Alterations in Light Exposure.

Application : WB

Reactivity : Zebrafish

Submit a Reference

REVIEW

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Application Reference

Moore NS et al. Cells 2021; 10 (3) Critical Effects on Akt Signaling in Adult Zebrafish Brain Following Alterations in Light Exposure.

Application : WB

Reactivity : Zebrafish

SDS
PBS.pdf
Sodium Azide.pdf

Package	List Price ($)
100 μl	$ 399
Qty

Hsp70 antibody [2A4]

Cat. No. GTX25442

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX25442 IHC-P Image

GTX25442 ICC/IF Image

GTX25442 ICC/IF Image

GTX25442 IP Image

GTX25442 ICC/IF Image

GTX25442 IHC-P Image

GTX25442 ICC/IF Image

GTX25442 WB Image

GTX25442 WB Image

GTX25442 IHC-P Image

GTX25442 ICC/IF Image

GTX25442 ICC/IF Image

GTX25442 ICC/IF Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.