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Hsp70 antibody [3A3]

Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunoprecipitation (IP). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439

Cat. No. GTX25439

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA, Gel supershift assays, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Yeast, Drosophila, Pig, Monkey, Amphibians, Echinoderms, Plant, Fish, Arthropod, Mollusc, Primate, Avian, Insect
Package
50 μl ($399)
See all Hsp70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
ICC/IF 1:100
IHC-P 1:200
FACS Assay dependent
IP 2 μl
ELISA Assay dependent
Gel supershift assays Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Product Note

This antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, p75, and following heat shock, HSP72 from yeast, Drosophila, fish, porcine, plant, mouse, avian, arthropod (red claw crayfish and black tiger prawn), amphibian, non-human primate, and human samples. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant HSP70 overexpressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_373941

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439

GTX25439 WB Image

WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000

Anti-Hsp70 antibody [3A3] used in Immunoprecipitation (IP). GTX25439

GTX25439 IP Image

IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439

GTX25439 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439

GTX25439 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439

GTX25439 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439

GTX25439 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439

GTX25439 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439

GTX25439 IHC-P Image

IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439

GTX25439 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439

GTX25439 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000

REFERENCE

Application Reference

Avenda?o-Borromeo B et al. Parasitol Res 2019; 118 (2) : 517-538 Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica.
Application : ICC/IF
Chang CC et al. Obesity (Silver Spring) 2017; (Epub) Caveolin-1 Secreted from Adipose Tissues and Adipocytes Functions as an Adipogenesis Enhancer.
Application : WB
Reactivity : Mouse
Barreca MM et al. J Cell Physiol 2016; Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway.
Application : WB
Reactivity : Mouse
Fang CT et al. Cell Mol Life Sci 2016; (Epub) HSP70 regulates the function of mitotic centrosomes.
Application : IP
Reactivity : Human
Ozaydin T et al. Microsc Res Tech 2016; Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages.
Application : IHC
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399