Hsp70 antibody [3A3]

Cat. No. GTX25439
8
Cat. No.  GTX25439
Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439
GTX25439 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000

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Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
GTX25439 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

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Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
GTX25439 IHC-P Image

IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

3 / 10
Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
GTX25439 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

4 / 10
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
GTX25439 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

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Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
GTX25439 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

6 / 10
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
GTX25439 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

7 / 10
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
GTX25439 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

8 / 10
Anti-Hsp70 antibody [3A3] used in Immunoprecipitation (IP). GTX25439
GTX25439 IP Image

IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

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Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439
GTX25439 WB Image

WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000

10 / 10
Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439
Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
Anti-Hsp70 antibody [3A3] used in IHC (Paraffin sections) (IHC-P). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25439
Anti-Hsp70 antibody [3A3] used in Immunoprecipitation (IP). GTX25439
Anti-Hsp70 antibody [3A3] used in Western Blot (WB). GTX25439
This Hsp70 antibody [3A3] (GTX25439) is a mouse monoclonal antibody validated for detection of Hsp70 by WB, ICC/IF, IHC-P, FCM, IP, ELISA, Gel supershift assays, IHC, and Neutralizing /Inhibition. Reactivity has been shown in Human, Mouse, Rat, and other species.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    3A3
  • Isotype
    IgG1
  • Applications
    WB ICC/IF IHC-P FCM IP ELISA Gel supershift assays IHC Neutralizing /Inhibition
  • Reactivity
    Human, Mouse, Rat, Yeast, Drosophila, Pig, Monkey, Amphibians, Echinoderms, Plant, Fish, Arthropod, Mollusc, Primate, Avian, Insect
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