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Hsp70 antibody [3C6]

Anti-Hsp70 antibody [3C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84338
Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338
Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338
Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338
Anti-Hsp70 antibody [3C6] used in Flow cytometry (FACS). GTX84338
Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338

Cat No. GTX84338

Host

Mouse

Clonality

Monoclonal

Clone Name

3C6

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Dog, Monkey
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Product Note

This antibody is able to recognize Hsp70 protein , which includes HSPA1A and HSPA1B protein.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human Hsp70 produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody [3C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84338

GTX84338 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Hsp70 plasmid using GTX84338 Hsp70 antibody [3C6].

Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338

GTX84338 WB Image

WB analysis of HEK293T cells transfected with Hsp70 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84338 Hsp70 antibody [3C6].
Loading : 5 ug per lane

Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338

GTX84338 WB Image

WB analysis of various cell lines using GTX84338 Hsp70 antibody [3C6].
Loading : 35 ug per lane

Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338

GTX84338 IHC-P Image

IHC-P analysis of human liver tissue using GTX84338 Hsp70 antibody [3C6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Hsp70 antibody [3C6] used in Flow cytometry (FACS). GTX84338

GTX84338 FACS Image

FACS analysis of HeLa cells using GTX84338 Hsp70 antibody [3C6].
Red : Primary antibody
Blue : Negative control antibody

Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338

GTX84338 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84338 Hsp70 antibody [3C6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf