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Hsp70 antibody [4E7]

Anti-Hsp70 antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57551
Anti-Hsp70 antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX57551
Anti-Hsp70 antibody [4E7] used in Western Blot (WB). GTX57551

Cat. No. GTX57551

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all HSPA1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
IHC-P 1:100-1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 4E7 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp72 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , HELS103 , HSP70.1 , HSP701 , HSP701A , HSP70I , HSP72 , HSPA1 , HSPA1A , HSC70

Cellular Localization

Cytoplasm

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57551

GTX57551 ICC/IF Image

ICC/IF analysis of A549 cells using GTX57551 HSPA1A antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-Hsp70 antibody [4E7] used in IHC (Paraffin sections) (IHC-P). GTX57551

GTX57551 IHC-P Image

IHC-P analysis of human eccrine gland using GTX57551 HSPA1A antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:100

Anti-Hsp70 antibody [4E7] used in Western Blot (WB). GTX57551

GTX57551 WB Image

WB analysis of various samples using GTX57551 HSPA1A antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399