IHC-P analysis of human tonsil tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

ICC/IF analysis of NIH-3T3 cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of HeLa cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of NCI-H1299 Cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

IP analysis of HeLa cell lysates using GTX25444 Hsp70 antibody [4G4].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

WB analysis of 50 ug of the indicated whole cell lysates using GTX25444 Hsp70 antibody [4G4].
Dilution : 1:1000

IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

IHC-P analysis of human breast tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50