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Hsp70 antibody [4G4]

Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
Anti-Hsp70 antibody [4G4] used in Western Blot (WB). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunoprecipitation (IP). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444

Cat. No. GTX25444

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, Cytotoxic Assay, IHC, IM, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Primate
Package
50 μl ($399)
See all Hsp70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
ICC/IF 1:100
IHC-P 1:20 - 50
FACS 1:100 - 1:200
IP 1:1000
Cytotoxic Assay Assay dependent
IHC Assay dependent
IM Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Product Note

This antibody specifically detects HSP70 and does not cross-react with other members of the HSP70 family. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444

GTX25444 IHC-P Image

IHC-P analysis of human breast tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444

GTX25444 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Hsp70 antibody [4G4] used in Western Blot (WB). GTX25444

GTX25444 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX25444 Hsp70 antibody [4G4].
Dilution : 1:1000

Anti-Hsp70 antibody [4G4] used in Immunoprecipitation (IP). GTX25444

GTX25444 IP Image

IP analysis of HeLa cell lysates using GTX25444 Hsp70 antibody [4G4].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444

GTX25444 ICC/IF Image

ICC/IF analysis of NCI-H1299 Cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444

GTX25444 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444

GTX25444 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444

GTX25444 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399