Hsp70 antibody [4G4]

Cat. No. GTX25444
Cat. No.  GTX25444
Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
GTX25444 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

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Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
GTX25444 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

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Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
GTX25444 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

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Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
GTX25444 ICC/IF Image

ICC/IF analysis of NCI-H1299 Cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

4 / 8
Anti-Hsp70 antibody [4G4] used in Immunoprecipitation (IP). GTX25444
GTX25444 IP Image

IP analysis of HeLa cell lysates using GTX25444 Hsp70 antibody [4G4].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

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Anti-Hsp70 antibody [4G4] used in Western Blot (WB). GTX25444
GTX25444 WB Image

WB analysis of 50 ug of the indicated whole cell lysates using GTX25444 Hsp70 antibody [4G4].
Dilution : 1:1000

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Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
GTX25444 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

7 / 8
Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
GTX25444 IHC-P Image

IHC-P analysis of human breast tissue using GTX25444 Hsp70 antibody [4G4].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

8 / 8
Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25444
Anti-Hsp70 antibody [4G4] used in Immunoprecipitation (IP). GTX25444
Anti-Hsp70 antibody [4G4] used in Western Blot (WB). GTX25444
Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
Anti-Hsp70 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX25444
This Hsp70 antibody [4G4] (GTX25444) is a mouse monoclonal antibody validated for detection of Hsp70 by WB, ICC/IF, IHC-P, FCM, IP, Cytotoxic Assay, IHC, IM, and Neutralizing /Inhibition. Reactivity has been shown in Human, Mouse, and Primate.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    4G4
  • Isotype
    IgG1
  • Applications
    WB ICC/IF IHC-P FCM IP Cytotoxic Assay IHC IM Neutralizing /Inhibition
  • Reactivity
    Human, Mouse, Primate
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