Hsp70 antibody [5A5]

Cat. No. GTX22787
1
Cat. No.  GTX22787
Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
GTX22787 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

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Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
GTX22787 IHC-P Image

IHC-P analysis of human testis tissue using GTX22787 Hsp70 antibody [5A5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

2 / 9
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
GTX22787 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

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Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
GTX22787 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

4 / 9
Anti-Hsp70 antibody [5A5] used in Immunoprecipitation (IP). GTX22787
GTX22787 IP Image

IP analysis of HeLa cell lysates using GTX22787 Hsp70 antibody [5A5].
GTX22787 was used as IP and IB antibody
IP reaction : 2μg antibody / 500μg lysate
WB antibody Dilution : 1:1000

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Anti-Hsp70 antibody [5A5] used in Western Blot (WB). GTX22787
GTX22787 WB Image

WB analysis of 50μg of the indicated whole cell lysates using GTX22787 Hsp70 antibody [5A5].
Dilution : 1:1000

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Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
GTX22787 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX22787 Hsp70 antibody [5A5].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

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Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
GTX22787 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

8 / 9
Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
GTX22787 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22787 Hsp70 antibody [5A5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

9 / 9
Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunoprecipitation (IP). GTX22787
Anti-Hsp70 antibody [5A5] used in Western Blot (WB). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
This Hsp70 antibody [5A5] (GTX22787) is a mouse monoclonal antibody validated for detection of Hsp70 by WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, Gel supershift assays, IHC, and Neutralizing /Inhibition. Reactivity has been shown in Human, Mouse, Rat, and other species.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    5A5
  • Isotype
    IgG1
  • Applications
    WB ICC/IF IHC-P IHC-Fr IP ELISA Gel supershift assays IHC Neutralizing /Inhibition
  • Reactivity
    Human, Mouse, Rat, Yeast, Rabbit, Drosophila, Bovine, Amphibians, Fish, Mollusc, Flatworm, Avian
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