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Hsp70 antibody [5A5]

Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in Western Blot (WB). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunoprecipitation (IP). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787
Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787
Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787

Cat. No. GTX22787

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, Gel supershift assays, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Yeast, Rabbit, Drosophila, Bovine, Amphibians, Fish, Mollusc, Flatworm, Avian
Package
250 μl ($399)
See all Hsp70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250 - 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
ELISA Assay dependent
Gel supershift assays Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Product Note

This antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, GRP78, and following heat shock, HSP72. It is the first monoclonal antibody reported to react with the ATP binding region of HSP70 and an epitope in the amino terminus of HSP70.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787

GTX22787 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22787 Hsp70 antibody [5A5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787

GTX22787 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787

GTX22787 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX22787 Hsp70 antibody [5A5].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-Hsp70 antibody [5A5] used in Western Blot (WB). GTX22787

GTX22787 WB Image

WB analysis of 50μg of the indicated whole cell lysates using GTX22787 Hsp70 antibody [5A5].
Dilution : 1:1000

Anti-Hsp70 antibody [5A5] used in Immunoprecipitation (IP). GTX22787

GTX22787 IP Image

IP analysis of HeLa cell lysates using GTX22787 Hsp70 antibody [5A5].
GTX22787 was used as IP and IB antibody
IP reaction : 2μg antibody / 500μg lysate
WB antibody Dilution : 1:1000

Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787

GTX22787 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Hsp70 antibody [5A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22787

GTX22787 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787

GTX22787 IHC-P Image

IHC-P analysis of human testis tissue using GTX22787 Hsp70 antibody [5A5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Hsp70 antibody [5A5] used in IHC (Paraffin sections) (IHC-P). GTX22787

GTX22787 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

REFERENCE

Application Reference

Chamberlain KA et al. Neuron 2021; (Epub) Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399