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Hsp70 antibody [HL1580]

Recombinant antibody Comparison
Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059
Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059
Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059
Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059
Anti-Hsp70 antibody [HL1580] used in IHC (Paraffin sections) (IHC-P). GTX637059
Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059
Anti-Hsp70 antibody [HL1580] used in IHC (Paraffin sections) (IHC-P). GTX637059
Anti-Hsp70 antibody [HL1580] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637059

Cat. No. GTX637059

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1580

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Hsp70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Observed MW

70 kDa.

Predict Reactivity

Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hsp70. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059

GTX637059 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Hsp70 antibody [HL1580] (GTX637059) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059

GTX637059 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Hsp70 antibody [HL1580] (GTX637059) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059

GTX637059 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody [HL1580] (GTX637059) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059

GTX637059 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody [HL1580] (GTX637059) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody [HL1580] used in IHC (Paraffin sections) (IHC-P). GTX637059

GTX637059 IHC-P Image

Hsp70 antibody [HL1580] detects Hsp70 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Hsp70 stained by Hsp70 antibody [HL1580] (GTX637059) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp70 antibody [HL1580] used in Western Blot (WB). GTX637059

GTX637059 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody [HL1580] (GTX637059) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody [HL1580] used in IHC (Paraffin sections) (IHC-P). GTX637059

GTX637059 IHC-P Image

Hsp70 antibody [HL1580] detects Hsp70 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Hsp70 stained by Hsp70 antibody [HL1580] (GTX637059) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp70 antibody [HL1580] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637059

GTX637059 ICC/IF Image

Hsp70 antibody [HL1580] detects Hsp70 protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hsp70 stained by Hsp70 antibody [HL1580] (GTX637059) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations