FACS/IF: Use at an assay dependent dilution. IP(Native and denatured): Use at a concentration of 2μg/mg. WB: Use at a concentration of 0.5-1μg/ml for 2hrs at RT. This antibody detects a band of 73 kDa (constitutive), 72 kDa(inducible), which corresponds to the predicted molecular weight of Hsp 70. IHC(formalin fixed paraffin embedded): Use at a concentration of 2-4μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.
BT474 or HeLa cells. Breast carcinoma.
Recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70.
Phosphate-buffered saline, pH 7.2, containing 0.2%BSA, 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
HSP70 protein from HeLa cells.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70