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Hsp70 antibody [W27]

Cat No. GTX23148

Host

Mouse

Clonality

Monoclonal

Clone Name

W27

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Bovine, Monkey
Package
500 μl ($319)

APPLICATION

Application Note

FACS/IF: Use at an assay dependent dilution. IP(Native and denatured): Use at a concentration of 2μg/mg. WB: Use at a concentration of 0.5-1μg/ml for 2hrs at RT. This antibody detects a band of 73 kDa (constitutive), 72 kDa(inducible), which corresponds to the predicted molecular weight of Hsp 70. IHC(formalin fixed paraffin embedded): Use at a concentration of 2-4μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

BT474 or HeLa cells. Breast carcinoma.

Product Note

Recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70.

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.2%BSA, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HSP70 protein from HeLa cells.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384957

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf