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Hsp70 antibody [W27]

Cat. No. GTX23148

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 W27

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Bovine, Monkey
Package
500 μl ($319)
See all Hsp70 products

APPLICATION

Application Note

FACS/IF: Use at an assay dependent dilution. IP(Native and denatured): Use at a concentration of 2μg/mg. WB: Use at a concentration of 0.5-1μg/ml for 2hrs at RT. This antibody detects a band of 73 kDa (constitutive), 72 kDa(inducible), which corresponds to the predicted molecular weight of Hsp 70. IHC(formalin fixed paraffin embedded): Use at a concentration of 2-4μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

BT474 or HeLa cells. Breast carcinoma.

Product Note

Recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HSP70 protein from HeLa cells.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384957

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

REFERENCE

Application Reference

Lamottke B et al. Eur J Haematol 2012; 88 (5) : 406-15 The novel, orally bioavailable HSP90 inhibitor NVP-HSP990 induces cell cycle arrest and apoptosis in multiple myeloma cells and acts synergistically with * by increased cleavage of caspases.
Application : FACS
Reactivity : Human
Kaiser M et al. Blood Cancer J 2011; 1 (7) : e28 Antileukemic activity of the HSP70 inhibitor pifithrin-μ in acute leukemia.
Application : FACS
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319