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Hsp70 antibody

Anti-Hsp70 antibody used in Western Blot (WB). GTX106148
Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106148
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX106148
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX106148

Cat No. GTX106148

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:500-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hsp70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950520

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody used in Western Blot (WB). GTX106148

GTX106148 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody (GTX106148) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106148

GTX106148 ICC/IF Image

Hsp70 antibody detects Hsp70 protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hsp70 protein stained by Hsp70 antibody (GTX106148) diluted at 1:400.
Blue: Hoechst 33342 staining.

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX106148

GTX106148 IHC-P Image

Hsp70 antibody detects Hsp70 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hsp70 antibody (GTX106148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX106148

GTX106148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human glioma, using Hsp70 (GTX106148) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169