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Hsp70 antibody

Anti-Hsp70 antibody used in Immunoprecipitation (IP). GTX111088
Anti-Hsp70 antibody used in Western Blot (WB). GTX111088
Anti-Hsp70 antibody used in Western Blot (WB). GTX111088
Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111088
Anti-Hsp70 antibody used in Western Blot (WB). GTX111088
Anti-Hsp70 antibody used in Western Blot (WB). GTX111088
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX111088
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX111088

Cat No. GTX111088

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , NIH-3T3 , Mouse brain , PC-12 , Rat brain , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , *Mesenchymal stem cells

Predict Reactivity

Sheep, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hsp70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950518

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody used in Immunoprecipitation (IP). GTX111088

GTX111088 IP Image

Hsp70 antibody immunoprecipitates Hsp70 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Hsp70 protein by 2.5 μg of Hsp70 antibody (GTX111088) 12% SDS-PAGE The immunoprecipitated Hsp70 protein was detected by Hsp70 antibody (GTX111088) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Hsp70 antibody used in Western Blot (WB). GTX111088

GTX111088 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX111088 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in Western Blot (WB). GTX111088

GTX111088 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111088 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111088

GTX111088 ICC/IF Image

Hsp70 antibody detects Hsp70 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hsp70 stained by Hsp70 antibody (GTX111088) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Hsp70 antibody used in Western Blot (WB). GTX111088

GTX111088 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: NIH-3T3
C: Mouse brain
D: PC-12
E: Rat brain
7.5% SDS PAGE
GTX111088 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in Western Blot (WB). GTX111088

GTX111088 WB Image

Various whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody (GTX111088) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX111088

GTX111088 IHC-P Image

Hsp70 antibody detects Hsp70 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hsp70 antibody (GTX111088) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX111088

GTX111088 IHC-P Image

Hsp70 antibody detects Hsp70 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Hsp70 antibody (GTX111088) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Hsp70 antibody Cat No. GTX111088

Rating ( Average 4 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4 based on 5 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Mk
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 26863132
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : skeletal muscle tissue
Amount used : 60μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Lane Description : Lane 1: mouse skeletal muscle tissue (3-month-old), Lane 2: mouse skeletal muscle tissue (24-month-old)
Date : Anonymous submitted on 03-Feb-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 20μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:500, 25°C, 2Hr
Date : Anonymous submitted on 07-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Mesenchymal stem cells
Amount used : 50μg
Blocking : 10% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane1: Mesenchymal stem cells (24hr) Lane2: Mesenchymal stem cells (48hr)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf