APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , Mouse brain , PC-12 , Rat brain , 293T
Predict Reactivity
Sheep, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Hsp70. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950518
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70
Background
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX111088 IP Image
Hsp70 antibody immunoprecipitates Hsp70 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Hsp70 protein by 2.5 μg of Hsp70 antibody (GTX111088) 12% SDS-PAGE The immunoprecipitated Hsp70 protein was detected by Hsp70 antibody (GTX111088) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX111088 IHC-P Image
Hsp70 antibody detects Hsp70 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hsp70 antibody (GTX111088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111088 WB Image
Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111088 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111088 ICC/IF Image
Hsp70 antibody detects Hsp70 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Hsp70 stained by Hsp70 antibody (GTX111088) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX111088 IHC-P Image
Hsp70 antibody detects Hsp70 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Hsp70 antibody (GTX111088) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111088 IP Image
Immunoprecipitation of Hsp70 protein from HeLa whole cell extract using 5 μg of Hsp70 antibody (GTX111088).
Western blot analysis was performed using Hsp70 antibody (GTX111088).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).
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GTX111088 WB Image
Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX111088 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111088 WB Image
Various whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody (GTX111088) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111088 WB Image
Sample (30 μg of whole cell lysate)
A: 293T
B: NIH-3T3
C: Mouse brain
D: PC-12
E: Rat brain
7.5% SDS PAGE
GTX111088 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111088 IHC-P Image
HSPA1A antibody detects HSPA1A protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human stomach.HSPA1A stained by HSPA1A antibody (GTX111088) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111088 WB Image
The data was published in the journal PLoS One in 2016.PMID: 26863132
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REFERENCE
REVIEW
Hsp70 antibody
Cat. No. GTX111088
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Rating
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( Average 4 based on 5 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 5 users reviews)
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Date :
Anonymous submitted on 11-Mar-2024
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse brown adipose tissue
Amount used :
20μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:8000, 4°C, >12Hr
Date :
Anonymous submitted on 08-Jan-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
PANC1 cells
Amount used :
25 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 29-Apr-2016
Application Tested :
WB
Sample Species :
Ms
Sample :
skeletal muscle tissue
Amount used :
60μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:10000, 4°C, 16Hr
Lane Description :
Lane 1: mouse skeletal muscle tissue (3-month-old), Lane 2: mouse skeletal muscle tissue (24-month-old)
Date :
Anonymous submitted on 03-Feb-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
MDA-MB-231 cells
Amount used :
20μg
Blocking :
5% milk, 37°C, 1Hr
Primary Antibody :
1:500, 25°C, 2Hr
Date :
Anonymous submitted on 07-Jan-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
Mesenchymal stem cells
Amount used :
50μg
Blocking :
10% milk, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr
Lane Description :
Lane1: Mesenchymal stem cells (24hr) Lane2: Mesenchymal stem cells (48hr)
