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Hsp70 antibody

Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11573
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573
Anti-Hsp70 antibody used in IHC (Frozen sections) (IHC-Fr). GTX11573
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573
Anti-Hsp70 antibody used in Western Blot (WB). GTX11573

Cat No. GTX11573

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

2.5% BSA, 0.45% NaCl, 0.1% Na₂HPO₄, 0.025% Thimerosal, 0.025% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70(13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885391

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11573

GTX11573 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573

GTX11573 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in IHC (Frozen sections) (IHC-Fr). GTX11573

GTX11573 IHC-Fr Image

IHC-Fr analysis of rat intestine tissue using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573

GTX11573 IHC-P Image

IHC-P analysis of rat brain tissue using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in Western Blot (WB). GTX11573

GTX11573 WB Image

WB analysis of various samples using GTX11573 Hsp70 antibody.
Lane 1 : rat testis tissue lysates
Lane 2 : rat brain tissue lysates
Lane 3 : rat liver tissue lysates
Lane 4 : rat lung tissue lysates
Lane 5 : CEM whole cell lysates
Lane 6 : HeLa whole cell lysates
Lane 7 : SMMC whole cell lysates
Lane 8 : HT1080 whole cell lysates
Lane 9 : COLO320 whole cell lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf