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Hsp70 antibody

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573
Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11573
Anti-Hsp70 antibody used in IHC (Frozen sections) (IHC-Fr). GTX11573
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573
Anti-Hsp70 antibody used in Western Blot (WB). GTX11573

Cat. No. GTX11573

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all Hsp70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70(13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885391

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573

GTX11573 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11573

GTX11573 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in IHC (Frozen sections) (IHC-Fr). GTX11573

GTX11573 IHC-Fr Image

IHC-Fr analysis of rat intestine tissue using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX11573

GTX11573 IHC-P Image

IHC-P analysis of rat brain tissue using GTX11573 Hsp70 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Hsp70 antibody used in Western Blot (WB). GTX11573

GTX11573 WB Image

WB analysis of various samples using GTX11573 Hsp70 antibody.
Lane 1 : rat testis tissue lysates
Lane 2 : rat brain tissue lysates
Lane 3 : rat liver tissue lysates
Lane 4 : rat lung tissue lysates
Lane 5 : CEM whole cell lysates
Lane 6 : HeLa whole cell lysates
Lane 7 : SMMC whole cell lysates
Lane 8 : HT1080 whole cell lysates
Lane 9 : COLO320 whole cell lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

REFERENCE

Application Reference

Liu YM et al. Eur J Clin Microbiol Infect Dis 2020; (Epub) miR-1975 serves as an indicator of clinical severity upon influenza infection.
Application : WB
Reactivity : Human
Liu YM et al. J Biomed Sci 2019; 26 (1) : 58 Exosome-delivered and Y RNA-derived small RNA suppresses influenza virus replication.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449