GeneTex

  • Country / Location Selection

United States (US)

Hsp90 alpha antibody [5G5]

Anti-Hsp90 alpha antibody [5G5] used in IHC (Paraffin sections) (IHC-P). GTX60587
Anti-Hsp90 alpha antibody [5G5] used in Flow cytometry (FACS). GTX60587
Anti-Hsp90 alpha antibody [5G5] used in ELISA (ELISA). GTX60587
Anti-Hsp90 alpha antibody [5G5] used in Western Blot (WB). GTX60587
Anti-Hsp90 alpha antibody [5G5] used in Western Blot (WB). GTX60587
Anti-Hsp90 alpha antibody [5G5] used in IHC (Paraffin sections) (IHC-P). GTX60587

Cat. No. GTX60587

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G5

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Monkey
Package
100 μg ($399)
See all Hsp90 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human HSP90AA1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887983

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 alpha family class A member 1 , EL52 , HEL-S-65p , HSP86 , HSP89A , HSP90A , HSP90N , HSPC1 , HSPCA , HSPCAL1 , HSPCAL4 , HSPN , Hsp103 , Hsp89 , Hsp90 , LAP-2 , LAP2

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Hsp90 alpha antibody [5G5] used in IHC (Paraffin sections) (IHC-P). GTX60587

GTX60587 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60587 Hsp90 alpha antibody [5G5].

Anti-Hsp90 alpha antibody [5G5] used in Flow cytometry (FACS). GTX60587

GTX60587 FACS Image

FACS analysis of HeLa cells using GTX60587 Hsp90 alpha antibody [5G5].
Green : Hsp90 alpha
Red : negative control

Anti-Hsp90 alpha antibody [5G5] used in ELISA (ELISA). GTX60587

GTX60587 ELISA Image

ELISA analysis of antigen using GTX60587 Hsp90 alpha antibody [5G5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Hsp90 alpha antibody [5G5] used in Western Blot (WB). GTX60587

GTX60587 WB Image

WB analysis of human Hsp90 alpha (AA: 275-484) recombinant protein using GTX60587 Hsp90 alpha antibody [5G5].

Anti-Hsp90 alpha antibody [5G5] used in Western Blot (WB). GTX60587

GTX60587 WB Image

WB analysis of NIH3T3 (1), HeLa (2), HCT116(3), HL-60 (4) and C0S7 (5) cell lysate using GTX60587 Hsp90 alpha antibody [5G5].

Anti-Hsp90 alpha antibody [5G5] used in IHC (Paraffin sections) (IHC-P). GTX60587

GTX60587 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60587 Hsp90 alpha antibody [5G5].

REFERENCE

Application Reference

Kumar P et al. Cancer Rep (Hoboken) 2020; : e1261 The matrix metalloproteinase 7 (MMP7) links Hsp90 chaperone with acquired drug resistance and tumor metastasis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Hsp90 alpha antibody [5G5] (GTX60587). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399