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Hsp90 alpha antibody

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX109753
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753
Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX109753
Anti-Hsp90 alpha antibody used in Immunoprecipitation (IP). GTX109753
Anti-Hsp90 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109753
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753
Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753

Cat No. GTX109753

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Monkey, Nematode
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain , *RK3E

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hsp90 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178231

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein 90 alpha family class A member 1 , EL52 , HEL-S-65p , HSP86 , HSP89A , HSP90A , HSP90N , HSPC1 , HSPCA , HSPCAL1 , HSPCAL4 , HSPN , Hsp103 , Hsp89 , Hsp90 , LAP-2 , LAP2

Cellular Localization

Cytoplasm , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX109753

GTX109753 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Hsp90 alpha antibody (GTX109753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp90 alpha antibody used in Western Blot (WB). GTX109753

GTX109753 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp90 alpha antibody used in Immunoprecipitation (IP). GTX109753

GTX109753 IP Image

Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 μg of Hsp90 alpha antibody (GTX109753).
Western blot analysis was performed using Hsp90 alpha antibody (GTX109753).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Hsp90 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109753

GTX109753 ICC/IF Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp90 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX109753

GTX109753 IHC-P Image

Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Hsp90 alpha antibody Cat No. GTX109753

Rating ( Average 5 based on 11 users reviews)
Application
Western Blot(WB) ( Average 5 based on 10 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2019.PMID: 31284899
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2019.PMID: 31284899
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 30846715
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2019.PMID: 31284899
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 30846715
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Mk
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 26863132
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cells in 2019.PMID: 31905895
Date : Anonymous submitted on 02-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC-3 cells
Blocking : 5% ECL Blocking Agent, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: WCL 10 μg, Lane 2: heat shock (43oC 1.5h) WCL 4 μg, Lane 3: heat shock (43oC 3.0h) WCL 8 μg
Date : Anonymous submitted on 26-Jul-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : MC3T3-E1 cells
Amount used : 50 μg
Blocking : 5% milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Date : Anonymous submitted on 26-Apr-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane1: HT29 shLacZ (virus infection to silence LacZ gene), Lane2: HT29 shHSP90AA1 ( virus infection to silence HSP90AA1 gene)
Date : Anonymous submitted on 18-Jan-2013
Score :
Application Tested : IP
Sample Species : Rat
Sample : RK3E cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Figure Note : 2ug for IP
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf