IHC-P analysis of differentiated colon adenocarcinoma tissue from human colon tissue sections using GTX22928 Hsp90 alpha antibody.
Left : without primary antibody
Right : with primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC
Dilution : 1:100

ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX22928 Hsp90 alpha antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22928 Hsp90 alpha antibody.
Dilution : 1:1000

IHC-P analysis of human tonsil tissue using GTX22928 Hsp90 alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

WB analysis of Hsp86 (Hsp90 alpha) and Hsp84 (Hsp90 beta) using GTX22928 Hsp90 alpha antibody. (left) or GTX79247 HSP90 beta antibody respectively

WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22928 Hsp90 alpha antibody.
Dilution : 1:1000

IP analysis of HeLa cell lysates using GTX22928 Hsp90 alpha antibody.
GTX22928 was used as IP and IB antibody
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

WB analysis of A431 cell lysate (Lane 3~6) using GTX22928 Hsp90 alpha antibody. Lanes 1 and 2 are control samples of purified, recombinant Hsp86 (Hsp90 alpha) and Hsp84 (Hsp90 beta), respectively.
Dilution : 1:2000

WB analysis of 50 ug of the indicated whole cell lysates using GTX22928 Hsp90 alpha antibody.
Hsp86 also known as Hsp90 alpha
Dilution : 1:1000

IHC-P analysis of human breast carcinoma tissue using GTX22928 Hsp90 alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

IHC-P analysis of human kidney tissue using GTX22928 Hsp90 alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100