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Hsp90 beta antibody [5G4]

Anti-Hsp90 beta antibody [5G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60458
Anti-Hsp90 beta antibody [5G4] used in Western Blot (WB). GTX60458
Anti-Hsp90 beta antibody [5G4] used in ELISA (ELISA). GTX60458
Anti-Hsp90 beta antibody [5G4] used in IHC (Paraffin sections) (IHC-P). GTX60458
Anti-Hsp90 beta antibody [5G4] used in Flow cytometry (FACS). GTX60458

Cat. No. GTX60458

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($409)
See all Hsp90 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human HSP90AB1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 alpha family class B member 1 , D6S182 , HSP84 , HSP90B , HSPC2 , HSPCB

Cellular Localization

Cytoplasm,Nucleus,Secreted,Cell membrane

Background

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-Hsp90 beta antibody [5G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60458

GTX60458 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60458 HSP90 beta antibody [5G4].
Green : HSP90 beta
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Hsp90 beta antibody [5G4] used in Western Blot (WB). GTX60458

GTX60458 WB Image

WB analysis of Jurkat (1), A431 (2), HeLa (3), A549 (4), HEK293 (5), K562 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60458 HSP90 beta antibody [5G4].

Anti-Hsp90 beta antibody [5G4] used in ELISA (ELISA). GTX60458

GTX60458 ELISA Image

ELISA analysis of antigen using GTX60458 HSP90 beta antibody [5G4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-Hsp90 beta antibody [5G4] used in IHC (Paraffin sections) (IHC-P). GTX60458

GTX60458 IHC-P Image

IHC-P analysis of kidney cancer tissue (left) and brain tissue (right) using GTX60458 HSP90 beta antibody [5G4].

Anti-Hsp90 beta antibody [5G4] used in Flow cytometry (FACS). GTX60458

GTX60458 FACS Image

FACS analysis of HeLa cells using GTX60458 HSP90 beta antibody [5G4].
Green : HSP90 beta
Purple : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409