APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLaS3 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
5.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP90 beta. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_1240970
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein 90 alpha family class B member 1 , D6S182 , HSP84 , HSP90B , HSPC2 , HSPCB
Cellular Localization
Cytoplasm , Melanosome
Background
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX101423 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Hsp90 beta antibody [N2C2], Internal (GTX101423) at 1:300 dilution.
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GTX101423 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101423 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101423 IHC-P Image
HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101423 WB Image
Sample(30 μg of whole cell lysate) A:HeLa S3(GTX14654) 7.5% SDS PAGE GTX101423 diluted at 1:2000
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GTX101423 IHC-P Image
HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101423 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta(GTX101423) antibody at 1:500 dilution.
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GTX101423 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Hsp83 is the homolog of mammalian Hsp90. (Gene ID: 38389/ UniProt: P02828)
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GTX101423 IHC-Wm Image
Hsp90 beta antibody [N2C2], Internal detects Hsp90 beta protein at cytosol on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Hsp90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:200.
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GTX101423 WB Image
Sample (30 μg of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE GTX101423 diluted at 1:1000
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REFERENCE
REVIEW
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