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Hsp90 beta antibody

KO/KD Orthogonal
Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101448
Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101448
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101448
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101448
Anti-Hsp90 beta antibody used in IHC (Whole mount) (IHC-Wm). GTX101448
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448

Cat. No. GTX101448

Reference 16
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, ELISA-Ag

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Hsp90 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP Assay dependent
ELISA-Ag Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human HSP90 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950515

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 alpha family class B member 1 , D6S182 , HSP84 , HSP90B , HSPC2 , HSPCB

Cellular Localization

Cytoplasm , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101448

GTX101448 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta(GTX101448) antibody at 1:500 dilution.

Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101448

GTX101448 IHC-P Image

HSP90 beta antibody detects HSP90 beta protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
HSP90 beta antibody (GTX101448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448

GTX101448 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody (GTX101448) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448

GTX101448 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody (GTX101448) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101448

GTX101448 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using HSP90 beta(GTX101448) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448

GTX101448 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
7.5% SDS PAGE
GTX101448 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101448

GTX101448 IHC-P Image

HSP90 beta antibody detects HSP90 beta protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
HSP90 beta antibody (GTX101448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hsp90 beta antibody used in IHC (Whole mount) (IHC-Wm). GTX101448

GTX101448 IHC-Wm Image

Hsp90 beta antibody detects Hsp90 beta protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Hsp90 beta antibody (GTX101448) dilution: 1:200.
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448

GTX101448 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX101448 diluted at 1:10000

Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448

GTX101448 WB Image

Orthogonal

The data was published in the 2018 in BMC Cancer. PMID: 30305041

REFERENCE

Application Reference

S Suzuki et al. J Dent Res 2023; The histone deacetylase inhibitor MS-275 enhances the matrix mineralization of dental pulp stem cells by inducing fibronectin expression
Application : WB
SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23) : 00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B
Application : WB, ICC/IF
Reactivity : Human
Morrow CS et al. Sci Rep 2022; 12 (1) : 15001 Adult fibroblasts use aggresomes only in distinct cell-states.
Navare AT et al. J Cell Biol 2022; 221 (11) Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.
Application : WB
Reactivity : Human
Ono K et al. J Extracell Vesicles 2020; 9 (1) : 1769373 Triple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer.
Show More +
Submit a Reference

REVIEW

Hsp90 beta antibody Cat. No. GTX101448

Rating
( Average 4.8 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 5 users reviews)
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Date : Anonymous submitted on 27-Feb-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 10 μg
Blocking : 5% ECL Blocking Agent, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: PC-3 cells, Lane 2: Du145 cells
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Date : Anonymous submitted on 26-Jul-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : MC3T3-E1 cells
Amount used : 50 μg
Blocking : 5% milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Date : Anonymous submitted on 24-Apr-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW480 cells
Amount used : 40 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane1-Control medium, Lane2- Low folate medium, Lane3- High folate medium
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Date : Anonymous submitted on 20-May-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : 4T1 cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX101448
Date : Anonymous submitted on 31-Jan-2013
Score :
Application Tested : WB
Sample Species : Rat
Sample : RK3E cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:250, 4°C, 16Hr
Submit a Review
Application Reference
S Suzuki et al. J Dent Res 2023; The histone deacetylase inhibitor MS-275 enhances the matrix mineralization of dental pulp stem cells by inducing fibronectin expression
Application : WB
SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23):00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B
Application : WB, ICC/IF
Reactivity : Human
Morrow CS et al. Sci Rep 2022; 12 (1):15001 Adult fibroblasts use aggresomes only in distinct cell-states.
Navare AT et al. J Cell Biol 2022; 221 (11) Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.
Application : WB
Reactivity : Human
Ono K et al. J Extracell Vesicles 2020; 9 (1):1769373 Triple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer.
Navare AT et al. bioRxiv 2020; Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.
Application : WB
Reactivity : Human
Lee EJ et al. JCI Insight 2020; 5 (7) Multiexon deletion alleles of ATF6 linked to achromatopsia.
Eguchi T et al. Cells 2020; 9 (3) Cell Stress Induced Stressome Release Including Damaged Membrane Vesicles and Extracellular HSP90 by Prostate Cancer Cells.
Application : WB
Reactivity : Human
Jividen K et al. BMC Cancer 2018; 18 (1):960 Genomic analysis of DNA repair genes and androgen signaling in prostate cancer.
Application : WB
Reactivity : Human
Fujiwara T et al. Oral Oncol 2018; 86:251-257 Carcinogenic epithelial-mesenchymal transition initiated by oral cancer exosomes is inhibited by anti-EGFR antibody cetuximab.
Application : WB
Reactivity : Human
Fujiwara T et al. Biochem Biophys Res Commun 2018; (Epub) Anti-EGFR antibody * is secreted by oral squamous cell carcinoma and alters EGF-driven mesenchymal transition.
Application : WB
Reactivity : Human
Ono K et al. J Cell Biochem 2018; (Epub) HSP-enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells.
Application : WB
Reactivity : Human
McKenna MK et al. Cancer Biol Ther 2015; 16 (7):1088-98 Anti-cancer activity of withaferin A in B-cell lymphoma.
Application : WB
Reactivity : Human
Kohl S et al. Nat Genet 2015; 47 (7):757-65 Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.
Lin PH et al. J Biomed Sci 2015; 22 (1):44 N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting.
Application : WB, IP
Reactivity : Human
Karam R et al. EMBO Rep 2015; 16 (5):599-609 The unfolded protein response is shaped by the NMD pathway.
Application : ELISA-Ag
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169