Hsp90 beta antibody

IP analysis of HeLa cell lysates using GTX79247 HSP90 beta antibody.
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

ICC/IF analysis of A2058 Cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

WB analysis of mouse HSP86 (Hsp90 alpha) or HSP84 (Hsp90 beta) and protein using GTX22928 Hsp90 alpha antibody (Lane 1) or GTX79247 HSP90 beta antibody (Lane 2).

IHC-P analysis of human breast carcinoma tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

IHC-P analysis of human placenta tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

FACS analysis of permeabilized, fixed HeLa cells using GTX79247 HSP90 beta antibody.
Blue histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 μg/ml incubated for 1 hour on ice

ICC/IF analysis of HeLa and NIH3T3 cells using GTX79247 HSP90 beta antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

WB analysis of 50μg of the indicated whole cell lysates per well using GTX79247 HSP90 beta antibody.
Dilution : 1:1000

IHC-P analysis of human tonsil tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

ICC/IF analysis of U251 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of HepG2 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC