APPLICATION
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with distilled water to a concentration of 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4).
Preservative
No preservative
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
C-terminal 6×His and Avi tagged; Gln18-Ser740 of human ACE2 (Accession #Q9BYF1)
Expression System
HEK293 cells
Purity
> 95 % by SDS-PAGE
Endotoxin
< 0.1 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
angiotensin I converting enzyme 2,ACE2,ACEH
Cellular Localization
Secreted,Cell membrane,Cytoplasm
Background
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX01550-pro Functional Assay Image
Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 4 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, His and Avi tag (GTX01550-pro) (0.64-10,000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
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GTX01550-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein, His and Avi tag protein (GTX01550-pro) (1.56-100 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
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GTX01550-pro Image
SDS-PAGE of GTX01550-pro Human ACE2 (ECD) protein, His and Avi tag.
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REFERENCE
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REVIEW
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