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Human ACE2 (ECD) protein, mouse IgG Fc tag (active)

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro
Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

Cat No. GTX135683-pro

Application

ELISA, Functional Assay, Lateral Flow

Species

Human
Package
100 μg ($499)

APPLICATION

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water. Lyophilized from PBS buffer.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution and aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

Human ACE2 of NP_068576.1 (Met1-Ser740) with the Fc region of mouse IgG1 at the C-terminus

Expression System

HEK293 cells

Purity

>90%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACEH

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Lateral Flow Image

SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins. The signal intensity of the test line (T) decreases due to competition by the neutralizing antibody, while the control line (C) demonstrates a valid test.

Neutralizing Antibody: Spike RBD antibody [HL1002] (GTX635791)
C Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: Anti-human ACE2 antibody (GTX635897)
T Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: SARS-CoV-2 Spike RBD protein

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro ELISA Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro ELISA Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 spike (trimer) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (13.72-10000 ng/mL). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) (2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; C.37 lambda variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.03-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Image

GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, AY.1 / Delta plus variant, His tag (delta plus variant) (GTX136333-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Human ACE2 (ECD) protein, mouse IgG Fc tag (active). GTX135683-pro

GTX135683-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant Spike S1 protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.351 beta variant; P.1 gamma variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

Human ACE2 (ECD) protein, mouse IgG Fc tag (active) Cat No. GTX135683-pro

Rating ( Average 5 based on 1 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Nov-2021
Score :
Application Tested : Lateral Flow
Figure Note : SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins.
Package List Price ($)
$ 499