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Human ACE2 (ECD) protein, His and Avi tag (active)

Human ACE2 (ECD) protein, His and Avi tag (active). GTX01550-pro
Human ACE2 (ECD) protein, His and Avi tag (active). GTX01550-pro
Human ACE2 (ECD) protein, His and Avi tag (active). GTX01550-pro

Cat. No. GTX01550-pro

Reference 2

Application

 ELISA, Functional Assay

Species

 Human
Package
100 μg ($499)
See all ACE2 products

APPLICATION

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4).

Preservative

No preservative

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal 6×His and Avi tagged; Gln18-Ser740 of human ACE2 (Accession #Q9BYF1)

Expression System

HEK293 cells

Purity

> 95 % by SDS-PAGE

Endotoxin

< 0.1 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensin I converting enzyme 2,ACE2,ACEH

Cellular Localization

Secreted,Cell membrane,Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human ACE2 (ECD) protein, His and Avi tag (active). GTX01550-pro

GTX01550-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 4 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, His and Avi tag (GTX01550-pro) (0.64-10,000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Human ACE2 (ECD) protein, His and Avi tag (active). GTX01550-pro

GTX01550-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein, His and Avi tag protein (GTX01550-pro) (1.56-100 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Human ACE2 (ECD) protein, His and Avi tag (active). GTX01550-pro

GTX01550-pro Image

SDS-PAGE of GTX01550-pro Human ACE2 (ECD) protein, His and Avi tag.

REFERENCE

Application Reference

TW Liu et al. J Ethnopharmacol 2024; 320 : 117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein
Kobayashi J et al. Influenza Other Respir Viruses 2023; 17 (2) : e13093 Use of the particle agglutination/particle agglutination inhibition test for antigenic analysis of SARS-CoV-2.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 499