GeneTex
  • Country / Location Selection

United States (US)

Human ALDH1A1 protein (active)

Human ALDH1A1 protein (active). GTX00155-pro
Human ALDH1A1 protein (active). GTX00155-pro
Human ALDH1A1 protein (active). GTX00155-pro

Cat No. GTX00155-pro

Application Functional Assay
Species Human
Package
10 μg ($319)
APPLICATION

Application Note

ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was in a dose dependent manner.

Observed MW

58 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser2~Ser501 (NP_000680.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aldehyde dehydrogenase 1 family member A1,ALDC,ALDH-E1,ALDH1,ALDH11,HEL-9,HEL-S-53e,HEL12,PUMB1,RALDH1

Background

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]

Database

  • Gene ID: 216 ALDH1A1
  • UniProt: P00352 ALDH1A1

Research Area

DATA IMAGES
Human ALDH1A1 protein (active). GTX00155-pro

GTX00155-pro Functional Assay Image

Functional ELISA analysis of GTX00155-pro Human ALDH1A1 protein (active) which can bind immobilized IL21 protein.

Human ALDH1A1 protein (active). GTX00155-pro

GTX00155-pro Image

WB analysis of GTX00155-pro Human ALDH1A1 protein (active).

Human ALDH1A1 protein (active). GTX00155-pro

GTX00155-pro Image

SDS-PAGE analysis of GTX00155-pro Human ALDH1A1 protein (active).

REFERENCE
There are currently no references for Human ALDH1A1 protein (active) (GTX00155-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human ALDH1A1 protein (active) (GTX00155-pro). Be the first to share your experience with this product.