GeneTex

  • Country / Location Selection

United States (US)

Human APRT protein, His tag

Human APRT protein, His tag. GTX00157-pro
Human APRT protein, His tag. GTX00157-pro
Human APRT protein, His tag. GTX00157-pro

Cat. No. GTX00157-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($359)
See all APRT protein products

APPLICATION

Application Note

Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was in a dose dependent manner.

Observed MW

23 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Glu180 (NP_000476.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

adenine phosphoribosyltransferase , AMP , APRTD

Background

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human APRT protein, His tag. GTX00157-pro

GTX00157-pro Functional Assay Image

Functional ELISA analysis of GTX00157-pro Human APRT protein which can bind immobilized VCAM1 protein.

Human APRT protein, His tag. GTX00157-pro

GTX00157-pro Image

WB analysis of GTX00157-pro Human APRT protein.

Human APRT protein, His tag. GTX00157-pro

GTX00157-pro Image

SDS-PAGE analysis of GTX00157-pro Human APRT protein.

REFERENCE

There are currently no references for Human APRT protein, His tag (GTX00157-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Human APRT protein, His tag (GTX00157-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Proclin.pdf
Package List Price ($)
$ 359