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Human APRT protein (active)

Human APRT protein (active). GTX00157-pro
Human APRT protein (active). GTX00157-pro
Human APRT protein (active). GTX00157-pro

Cat No. GTX00157-pro

Application Functional Assay
Species Human
Package
10 μg ($339)
APPLICATION

Application Note

Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was in a dose dependent manner.

Observed MW

23 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Glu180 (NP_000476.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

adenine phosphoribosyltransferase , AMP , APRTD

Background

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Human APRT protein (active). GTX00157-pro

GTX00157-pro Functional Assay Image

Functional ELISA analysis of GTX00157-pro Human APRT protein (active) which can bind immobilized VCAM1 protein.

Human APRT protein (active). GTX00157-pro

GTX00157-pro Image

WB analysis of GTX00157-pro Human APRT protein (active).

Human APRT protein (active). GTX00157-pro

GTX00157-pro Image

SDS-PAGE analysis of GTX00157-pro Human APRT protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 339