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Human ARMET protein, His tag (active)

Human ARMET protein, His tag (active). GTX00139-pro
Human ARMET protein, His tag (active). GTX00139-pro
Human ARMET protein, His tag (active). GTX00139-pro

Cat. No. GTX00139-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($269)
See all ARMET protein products

APPLICATION

Application Note

MANF (Mesencephalic astrocyte-derived neurotrophic factor) is a prosurvival protein that protects the cells when applied intracellularly in vitro or extracellularly in vivo. Recently, MANF has also been proven to inhibit cell proliferation and ER stress-induced cell death and also affects cell size and morphology under certain conditions. Thus the bioactivity of human recombinant MANF were detected using U87-MG. Briefly,  U87-MG cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of MANF. After incubated for 48h,  cells were observed by inverted microscope that MANF inhibited cell proliferation of U87-MG and affected cell size and morphology.

Observed MW

22 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly28~Leu182 (NP_006001.4)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mesencephalic astrocyte derived neurotrophic factor , ARMET , ARP

Background

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Human ARMET protein, His tag (active). GTX00139-pro

GTX00139-pro Functional Assay Image

Cell suppression effect of GTX00139-pro Human ARMET protein (active).
(A) Unstimulated U87-MG cells cultured in DMEM for 48hrs.
(B) U87-MG cells cultured in DMEM, stimulated with 0.01 ng/ml MANF for 48hrs.

Human ARMET protein, His tag (active). GTX00139-pro

GTX00139-pro Image

SDS-PAGE analysis of GTX00139-pro Human ARMET protein (active).

Human ARMET protein, His tag (active). GTX00139-pro

GTX00139-pro Image

WB analysis of GTX00139-pro Human ARMET protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 269