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Human ARSB protein (active)

Human ARSB protein (active). GTX00161-pro
Human ARSB protein (active). GTX00161-pro
Human ARSB protein (active). GTX00161-pro

Cat No. GTX00161-pro

Application

Functional Assay

Species

Human
Package
10 μg ($339)

APPLICATION

Application Note

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was in a dose dependent manner.

Observed MW

55 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu81~Met533 (NP_000037.2)

Expression System

293F cell

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

arylsulfatase B , ASB , G4S , MPS6

Background

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Human ARSB protein (active). GTX00161-pro

GTX00161-pro Functional Assay Image

Functional ELISA analysis of GTX00161-pro Human ARSB protein (active) which can bind immobilized uPAR protein.

Human ARSB protein (active). GTX00161-pro

GTX00161-pro Image

WB analysis of GTX00161-pro Human ARSB protein (active).

Human ARSB protein (active). GTX00161-pro

GTX00161-pro Image

SDS-PAGE analysis of GTX00161-pro Human ARSB protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 339