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Human ARSB protein (active)

Human ARSB protein (active). GTX00161-pro
Human ARSB protein (active). GTX00161-pro
Human ARSB protein (active). GTX00161-pro

Cat No. GTX00161-pro

Application Functional Assay
Species Human
Package
10 μg ($339)
APPLICATION

Application Note

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was in a dose dependent manner.

Observed MW

55 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu81~Met533 (NP_000037.2)

Expression System

293F cell

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

arylsulfatase B,ASB,G4S,MPS6

Background

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES
Human ARSB protein (active). GTX00161-pro

GTX00161-pro Functional Assay Image

Functional ELISA analysis of GTX00161-pro Human ARSB protein (active) which can bind immobilized uPAR protein.

Human ARSB protein (active). GTX00161-pro

GTX00161-pro Image

WB analysis of GTX00161-pro Human ARSB protein (active).

Human ARSB protein (active). GTX00161-pro

GTX00161-pro Image

SDS-PAGE analysis of GTX00161-pro Human ARSB protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 339