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Human ATG10 protein, His tag

Human ATG10 protein, His tag. GTX68788-pro

Cat No. GTX68788-pro

Species

Human
Package
100 μg ($289)

APPLICATION

Observed MW

25 kDa.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 0.1 M NaCl, 10% glycerol, 1 mM DTT.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, N-terminal His-Tag; MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 10 , APG10 , APG10L , pp12616

Cellular Localization

Cytoplasm

Background

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Human ATG10 protein, His tag. GTX68788-pro

GTX68788-pro Image

3μg Human ATG10 protein (GTX68788-pro) by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 289