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Human Adenylate kinase 2 protein, His tag (active)

Human Adenylate kinase 2 protein, His tag (active). GTX66907-pro

Cat No. GTX66907-pro

Application

Functional Assay

Species

Human
Package
100 μg ($289)

APPLICATION

Application Note

Specific activity is > 25 units/mg. One unit will convert 2.0 μmoles of ADP to ATP + AMP per minute at pH 7.5 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 2 mM DTT, 20% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 259 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

adenylate kinase 2 , ADK2

Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Human Adenylate kinase 2 protein, His tag (active). GTX66907-pro

GTX66907-pro Image

3 μg of GTX66907-pro Human Adenylate kinase 2 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 289