GeneTex
  • Country / Location Selection

United States (US)

Human Adiponectin Receptor 1 protein (active)

Human Adiponectin Receptor 1 protein (active). GTX00105-pro
Human Adiponectin Receptor 1 protein (active). GTX00105-pro
Human Adiponectin Receptor 1 protein (active). GTX00105-pro

Cat No. GTX00105-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

ADIPOR1 (Adiponectin receptor protein 1) is a receptor for ADP (30kDa adipocyte complement-related protein), an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Besides, human ADP and rat ADP exist similarities in amino acid sequence with the identity of 83.2% . Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ADIPOR1 and recombinant rat ADP. Briefly, ADIPOR1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ADP-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ADIPOR1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of ADIPOR1 and ADP was in a dose dependent manner.

Observed MW

63 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Met1~Gly136 (NP_001277482.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

adiponectin receptor 1,ACDCR1,CGI-45,CGI45,PAQR1,TESBP1A

Background

This gene encodes a protein which acts as a receptor for adiponectin, a hormone secreted by adipocytes which regulates fatty acid catabolism and glucose levels. Binding of adiponectin to the encoded protein results in activation of an AMP-activated kinase signaling pathway which affects levels of fatty acid oxidation and insulin sensitivity. A pseudogene of this gene is located on chromosome 14. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES
Human Adiponectin Receptor 1 protein (active). GTX00105-pro

GTX00105-pro Functional Assay Image

Functional ELISA analysis of GTX00105-pro Human Adiponectin Receptor 1 protein (active) which can bind immobilized ADP protein.

Human Adiponectin Receptor 1 protein (active). GTX00105-pro

GTX00105-pro Image

SDS-PAGE analysis of GTX00105-pro Human Adiponectin Receptor 1 protein (active).

Human Adiponectin Receptor 1 protein (active). GTX00105-pro

GTX00105-pro Image

WB analysis of GTX00105-pro Human Adiponectin Receptor 1 protein (active).

REFERENCE
There are currently no references for Human Adiponectin Receptor 1 protein (active) (GTX00105-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human Adiponectin Receptor 1 protein (active) (GTX00105-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 259