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Human Alkaline Phosphatase (Tissue Non-Specific) protein, His tag (active)

Human Alkaline Phosphatase (Tissue Non-Specific) protein, His tag (active). GTX03499-pro

Cat. No. GTX03499-pro

Application

 Functional Assay

Species

 Human
Package
50 μg ($369)
See all Alkaline Phosphatase protein products

APPLICATION

Application Note

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 50, 000 pmoles/min/μg.

Observed MW

65 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal polyhistidine tag; Met 1-Ser 502 (NP_000469.3)

Expression System

HEK293 Cells

Purity

> 95 % by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, biomineralization associated , AP-TNAP , APTNAP , HOPS , TNALP , TNAP , TNSALP

Cellular Localization

Cell membrane

Background

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Human Alkaline Phosphatase (Tissue Non-Specific) protein, His tag (active). GTX03499-pro

GTX03499-pro Image

SDS-PAGE of GTX03499-pro human Alkaline Phosphatase (Tissue Non-Specific) protein, His tag (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 369