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Human Amphiregulin protein (active)

Human Amphiregulin protein (active). GTX00421-pro
Human Amphiregulin protein (active). GTX00421-pro
Human Amphiregulin protein (active). GTX00421-pro

Cat No. GTX00421-pro

Application Functional Assay
Species Human
Package
10 μg ($229)
APPLICATION

Application Note

Amphiregulin, also known as AREG, is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. Besides, Epidermal Growth Factor Receptor (EGFR) has been identified as an interactor of ARGE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARGE and recombinant human EGFR. Briefly, ARGE were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EGFR-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ARGE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ARGE and EGFR was in a dose dependent manner.

Observed MW

44 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20 mM Tris (pH8.0), 150 mM NaCl to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag and GST-Tag; Ser20~Asp100 (NP_001648.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

amphiregulin , AR , AREGB , CRDGF , SDGF

Background

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES
Human Amphiregulin protein (active). GTX00421-pro

GTX00421-pro Functional Assay Image

Functional ELISA analysis of GTX00421-pro Human Amphiregulin protein (active) which can bind immobilized EGFR protein.

Human Amphiregulin protein (active). GTX00421-pro

GTX00421-pro Image

WB analysis of GTX00421-pro Human Amphiregulin protein (active).

Human Amphiregulin protein (active). GTX00421-pro

GTX00421-pro Image

SDS-PAGE analysis of GTX00421-pro Human Amphiregulin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 229