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Human Angiopoietin 2 protein, His tag

Human Angiopoietin 2 protein, His tag. GTX01319-pro
Human Angiopoietin 2 protein, His tag. GTX01319-pro

Cat No. GTX01319-pro

Application

Functional Assay

Species

Human
Package
50 μg ($279),
20 μg ($199)

APPLICATION

Application Note

Measured by its binding ability in a functional ELISA. Immobilized Human Angiopoietin 2 protein, His tag (active) (GTX01319-pro) at 2 μg/ml (100 μl/well) can bind human Tie2-his and human IgG1 protein. The EC₅₀ of human Tie2-his and human IgG1 protein is 0.60-1.2 μg/ml.

Observed MW

56.4 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Phe496 of Human Angiopoietin 2 protein (NP_001138.1)

Expression System

CHO cells

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AGPT2,ANG2

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human Angiopoietin 2 protein, His tag. GTX01319-pro

GTX01319-pro Functional Assay Image

The protein binding activity was measured in a functional ELISA analysis of human TIE2 protein which can bind immobilized GTX01319-pro Human Angiopoietin 2 protein.
Immobilized GTX01319-pro : 10 μg/ml (100 μl/well)
Human TIE2 protein : 0.6-1.2 μg/ml

Human Angiopoietin 2 protein, His tag. GTX01319-pro

GTX01319-pro Image

SDS-PAGE of 5 μg GTX01319-pro Human Angiopoietin 2 protein.

REFERENCE

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