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Human Angiopoietin 2 protein (active)

Human Angiopoietin 2 protein (active). GTX00212-pro
Human Angiopoietin 2 protein (active). GTX00212-pro
Human Angiopoietin 2 protein (active). GTX00212-pro

Cat No. GTX00212-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Angiopoietin is part of a family of vascular growth factors that play a role in embryonic and postnatal angiogenesis. Angiopoietin cytokines are involved with controlling microvascular permeability, vasodilation, and vasoconstriction by signaling smooth muscle cells surrounding vessels. There are now four identified angiopoietins: ANGPT1, ANGPT2, ANGPT3, ANGPT4. Angiopoietin 2 (ANGPT2) promotes cell death and disrupts vascularization. Yet, when it is in conjunction with vascular endothelial growth factors, or VEGF, it can promote neo-vascularization. Besides, TEK Tyrosine Kinase (Tie2) has been identified as an interactor of ANGPT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ANGPT2 and recombinant human Tie2. Briefly, ANGPT2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to Tie2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ANGPT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ANGPT2 and Tie2 was in a dose dependent manner.

Observed MW

18 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys24~Leu165 (NP_001112359.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

angiopoietin 2,AGPT2,ANG2

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human Angiopoietin 2 protein (active). GTX00212-pro

GTX00212-pro Functional Assay Image

Functional ELISA analysis of GTX00212-pro Human Angiopoietin 2 protein (active) which can bind immobilized Tie2 protein.

Human Angiopoietin 2 protein (active). GTX00212-pro

GTX00212-pro Image

SDS-PAGE analysis of GTX00212-pro Human Angiopoietin 2 protein (active).

Human Angiopoietin 2 protein (active). GTX00212-pro

GTX00212-pro Image

WB analysis of GTX00212-pro Human Angiopoietin 2 protein (active).

REFERENCE
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REVIEW
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