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Human Annexin A1 protein (active)

Human Annexin A1 protein (active). GTX00274-pro
Human Annexin A1 protein (active). GTX00274-pro
Human Annexin A1 protein (active). GTX00274-pro

Cat No. GTX00274-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2 -dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. Besides, S100 Calcium Binding Protein A11 (S100A11) has been identified as an interactor of ANXA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ANXA1 and recombinant human S100A11. Briefly, ANXA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A11-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ANXA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ANXA1 and S100A11 was in a dose dependent manner.

Observed MW

44 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Asn346 (NP_000691.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

annexin A1 , ANX1 , LPC1

Background

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES
Human Annexin A1 protein (active). GTX00274-pro

GTX00274-pro Functional Assay Image

Functional ELISA analysis of GTX00274-pro Human Annexin A1 protein (active) which can bind immobilized S100A11 protein.

Human Annexin A1 protein (active). GTX00274-pro

GTX00274-pro Image

SDS-PAGE analysis of GTX00274-pro Human Annexin A1 protein (active).

Human Annexin A1 protein (active). GTX00274-pro

GTX00274-pro Image

WB analysis of GTX00274-pro Human Annexin A1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259