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Human Annexin II protein (active)

Human Annexin II protein (active). GTX00273-pro
Human Annexin II protein (active). GTX00273-pro
Human Annexin II protein (active). GTX00273-pro

Cat No. GTX00273-pro

Application Functional Assay
Species Human
Package
10 μg ($229)
APPLICATION

Application Note

Annexin A2 (ANXA2) also known as annexin II is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. ANXA2 is up-regulated in various tumor types and plays multiple roles in regulating cellular functions, including angiogenesis, proliferation, apoptosis, cell migration, invasion and adhesion. To test the effect of ANXA2 on cell proliferation, Hela cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant human ANXA2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Hela cells after incubation with ANXA2 for 96h observed by inverted microscope. Cell viability was assessed by CCK-8 assay after incubation with recombinant ANXA2 for 96h. And ANXA2 significantly increased cell viability of Hela cells.

Observed MW

42 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Asp339 (NP_001002857.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

annexin A2,ANX2,ANX2L4,CAL1H,HEL-S-270,LIP2,LPC2,LPC2D,P36,PAP-IV

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human Annexin II protein (active). GTX00273-pro

GTX00273-pro Functional Assay Image

Cell proliferation effect of GTX00273-pro Human Annexin II protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated HeLa cells cultured in DMEM for 96hrs.
(B) HeLa cells cultured in DMEM, stimulated with 10 ng/ml ANXA2 for 96hrs.

Human Annexin II protein (active). GTX00273-pro

GTX00273-pro Image

SDS-PAGE analysis of GTX00273-pro Human Annexin II protein (active).

Human Annexin II protein (active). GTX00273-pro

GTX00273-pro Image

WB analysis of GTX00273-pro Human Annexin II protein (active).

REFERENCE
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