Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The unlabeled Annexin V reagent can be conjugated to any tag of choice for detection of apoptosis. The reagent can also be used in competition binding experiments to compete with tagged annexin V conjugates. Annexin V-FITC, -Cy3, -Cy5, -EGFP, -PE, PE/CY5, and -Biotin Labels are also available separately.
Lyophilized from PBS (pH 7.4). Reconstitute with ddH₂O or PBS to 1.0-5.0 mg/ml.
Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
≥ 98% by SDS-PAGE and HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]
Analytical Size Exclusion Chromatography run of Annexin V.