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Human Arylsulfatase F protein (active)

Human Arylsulfatase F protein (active). GTX00162-pro
Human Arylsulfatase F protein (active). GTX00162-pro
Human Arylsulfatase F protein (active). GTX00162-pro

Cat No. GTX00162-pro

Application

Functional Assay

Species

Human
Package
10 μg ($329)

APPLICATION

Application Note

ARSF (Arylsulfatase F) is a member of the sulfatase gene family, and more specifically, the arylsulfatase subfamily. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Besides, EP300 (E1A binding protein p300) has been identified as an interactor of ARSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSF and recombinant human EP300. Briefly, ARSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EP300-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ARSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of ARSF and EP300 was in a dose dependent manner.

Observed MW

35 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; His23~Asp300 (NP_001188467.1)

Expression System

293F cell

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

arylsulfatase F , ASF

Background

This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011]

Database

DATA IMAGES

Human Arylsulfatase F protein (active). GTX00162-pro

GTX00162-pro Functional Assay Image

Functional ELISA analysis of GTX00162-pro Human Arylsulfatase F protein (active) which can bind immobilized EP300 protein.

Human Arylsulfatase F protein (active). GTX00162-pro

GTX00162-pro Image

SDS-PAGE analysis of GTX00162-pro Human Arylsulfatase F protein (active).

Human Arylsulfatase F protein (active). GTX00162-pro

GTX00162-pro Image

WB analysis of GTX00162-pro Human Arylsulfatase F protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 329