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Human BAFF protein (active)

Human BAFF protein (active). GTX00260-pro
Human BAFF protein (active). GTX00260-pro
Human BAFF protein (active). GTX00260-pro

Cat No. GTX00260-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

Tumor necrosis factor ligand superfamily member 13B protein (TNFSF13B) also known as B-cell activating factor (BAFF) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Besides, Integrin Beta 1 (ITGb1) has been identified as an interactor of TNFSF13B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFSF13B and recombinant human ITGb1. Briefly, TNFSF13B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ITGb1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNFSF13B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNFSF13B and ITGb1 was in a dose dependent manner.

Observed MW

22 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys113~Lys283 (NP_001139117.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TNF superfamily member 13b,BAFF,BLYS,CD257,DTL,TALL-1,TALL1,THANK,TNFSF20,TNLG7A,ZTNF4

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Database

DATA IMAGES
Human BAFF protein (active). GTX00260-pro

GTX00260-pro Functional Assay Image

Functional ELISA analysis of GTX00260-pro Human BAFF protein (active) which can bind immobilized ITGb1 protein.

Human BAFF protein (active). GTX00260-pro

GTX00260-pro Image

WB analysis of GTX00260-pro Human BAFF protein (active).

Human BAFF protein (active). GTX00260-pro

GTX00260-pro Image

SDS-PAGE analysis of GTX00260-pro Human BAFF protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239