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Human CD137 protein, His tag

Human CD137 protein, His tag. GTX00128-pro
Human CD137 protein, His tag. GTX00128-pro
Human CD137 protein, His tag. GTX00128-pro

Cat. No. GTX00128-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($219)
See all CD137 protein products

APPLICATION

Application Note

TNFRSF9 (Tumor necrosis factor receptor superfamily member 9) is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. A binding ELISA assay was conducted to detect the interaction of TNFRSF9 and TNFa. Briefly, recombinant human TNFRSF9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNFRSF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF9 and TNFa was in a dose dependent manner.

Observed MW

16 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn40~Glu153 (NP_001552.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF receptor superfamily member 9 , 4-1BB , CD137 , CDw137 , ILA

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human CD137 protein, His tag. GTX00128-pro

GTX00128-pro Functional Assay Image

Functional ELISA analysis of GTX00128-pro Human CD137 protein which can bind immobilized TNF alpha protein.

Human CD137 protein, His tag. GTX00128-pro

GTX00128-pro Image

SDS-PAGE analysis of GTX00128-pro Human CD137 protein.

Human CD137 protein, His tag. GTX00128-pro

GTX00128-pro Image

WB analysis of GTX00128-pro Human CD137 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 219