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Human CD27 protein, His tag

Human CD27 protein, His tag. GTX00124-pro
Human CD27 protein, His tag. GTX00124-pro
Human CD27 protein, His tag. GTX00124-pro

Cat. No. GTX00124-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($239)
See all CD27 protein products

APPLICATION

Application Note

TNFRSF7 (Tumor necrosis factor receptor superfamily member 7), also known as CD27 antigen, is a member of the TNF-receptor superfamily. This receptor is thought to be involved in tumor necrosis factor-activated receptor activity by binding with TNFs. Thus, a binding ELISA assay was conducted to detect the association of TNFRSF7 with TNFa. Briefly, recombinant human TNFRSF7 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNFRSF7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF7 with TNFa was in a dose dependent manner.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser19~Asp188 (NP_001233.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD27 molecule , S152 , S152. LPFS2 , T14 , TNFRSF7 , Tp55

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human CD27 protein, His tag. GTX00124-pro

GTX00124-pro Functional Assay Image

Functional ELISA analysis of GTX00124-pro Human CD27 protein which can bind immobilized TNF alpha protein.

Human CD27 protein, His tag. GTX00124-pro

GTX00124-pro Image

SDS-PAGE analysis of GTX00124-pro Human CD27 protein.

Human CD27 protein, His tag. GTX00124-pro

GTX00124-pro Image

WB analysis of GTX00124-pro Human CD27 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 239