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Human CD40L / CD154 protein, DDDDK tag (active, multimeric)

Human CD40L / CD154 protein, DDDDK tag (active, multimeric). GTX65635-pro

Cat. No. GTX65635-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($409)
See all CD40L / CD154 protein products
PRODUCT

Summary

Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins.

APPLICATION

Application Note

Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED₅₀:: <1ng/ml).

Product Note

Binds to human CD40.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 100μl ddH₂O to 0.1mg/ml. Lyophilized from 0.2μm-filtered PBS.

Preservative

No preservative

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Region/Sequence

N-terminal DDDDK-Tag; Human CD40L (a.a.116-261) is fused at the N-terminus to mouse ACRP30headless (a.a.18-111).

Expression System

CHO cells

Purity

??95% by SDS-PAGE.

Endotoxin

< 0.01 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD40 ligand , CD154 , CD40L , HIGM1 , IGM , IMD3 , T-BAM , TNFSF5 , TRAP , gp39 , hCD40L

Database

  • Gene ID: 959 CD40LG

Research Area

DATA IMAGES

Human CD40L / CD154 protein, DDDDK tag (active, multimeric). GTX65635-pro

GTX65635-pro Image

GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100μl RPMI supplemented with 10% FCS) for 24 hours at 37ºC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1μg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 409