APPLICATION
Application Note
Cell proliferation of Raji cells after stimulated with GP39. Cell proliferation of Raji cells after stimulated with GP39. Glycoprotein 39, Cartilage (GP39) also known as Chitinase-3-like protein 1 (CHI3L1) is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. GP39 plays a role in cancer cell proliferation, survival, invasiveness and in the regulation of cell-matrix interactions. To test the effect of GP39 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 1% serum standard RPMI-1640 which contains various concentrations of recombinant human GP39. After incubated for 5 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Raji cells after incubation with GP39 for 5 days observed by inverted microscope.
Observed MW
26 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Asn112~Thr237+Ile299~Lys377 (NP_001267.2)
Expression System
E. coli
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
chitinase 3 like 1 , ASRT7 , CGP-39 , GP-39 , GP39 , HC-gp39 , HCGP-3P , YK-40 , YKL-40 , YKL40 , YYL-40 , hCGP-39
Background
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
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GTX00219-pro Functional Assay Image
Cell proliferation effect of GTX00219-pro Human CHI3L1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Raji cells cultured in RPMI-1640 for 5 days.
(B) Raji cells cultured in RPMI-1640, stimulated with 10 ng/ml GP39 for 5 days.
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GTX00219-pro Image
SDS-PAGE analysis of GTX00219-pro Human CHI3L1 protein (active).
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GTX00219-pro Image
WB analysis of GTX00219-pro Human CHI3L1 protein (active).
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REFERENCE
There are currently no references for Human CHI3L1 protein, His tag (active) (GTX00219-pro). Be the first to share your publications with this product.
REVIEW
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