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Human CHI3L1 protein (active)

Human CHI3L1 protein (active). GTX00219-pro
Human CHI3L1 protein (active). GTX00219-pro
Human CHI3L1 protein (active). GTX00219-pro

Cat No. GTX00219-pro

Application Functional Assay
Species Human
Package
10 μg ($299)
APPLICATION

Application Note

Cell proliferation of Raji cells after stimulated with GP39. Cell proliferation of Raji cells after stimulated with GP39. Glycoprotein 39, Cartilage (GP39) also known as Chitinase-3-like protein 1 (CHI3L1) is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. GP39 plays a role in cancer cell proliferation, survival, invasiveness and in the regulation of cell-matrix interactions. To test the effect of GP39 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 1% serum standard RPMI-1640 which contains various concentrations of recombinant human GP39. After incubated for 5 days,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Raji cells after incubation with GP39 for 5 days observed by inverted microscope.

Observed MW

26 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn112~Thr237+Ile299~Lys377 (NP_001267.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chitinase 3 like 1,ASRT7,CGP-39,GP-39,GP39,HC-gp39,HCGP-3P,YK-40,YKL-40,YKL40,YYL-40,hCGP-39

Background

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES
Human CHI3L1 protein (active). GTX00219-pro

GTX00219-pro Functional Assay Image

Cell proliferation effect of GTX00219-pro Human CHI3L1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Raji cells cultured in RPMI-1640 for 5 days.
(B) Raji cells cultured in RPMI-1640, stimulated with 10 ng/ml GP39 for 5 days.

Human CHI3L1 protein (active). GTX00219-pro

GTX00219-pro Image

WB analysis of GTX00219-pro Human CHI3L1 protein (active).

Human CHI3L1 protein (active). GTX00219-pro

GTX00219-pro Image

SDS-PAGE analysis of GTX00219-pro Human CHI3L1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 299