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Human CLCA1 protein (active)

Human CLCA1 protein (active). GTX00147-pro
Human CLCA1 protein (active). GTX00147-pro
Human CLCA1 protein (active). GTX00147-pro

Cat No. GTX00147-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

CLCA1 (Calcium-activated chloride channel regulator 1) is a member of the calcium sensitive chloride conductance protein family. This protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits. It has been reported that CLCA1 activates calcium-dependent chloride channel through the interaction with TMEM16A (anoctamin-1). Besides, there exits similarities between human and mouse TMEM16A in amino acid sequence with the identity of 89.66% . Thus, a functional ELISA assay was conducted to detect the association of recombinant human CLCA1 with recombinant mouse TMEM16A. Briefly, CLCA1 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TMEM16A-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CLCA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of CLCA1 with TMEM16A was in a dose dependent manner.

Observed MW

28 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn416~Arg670 (NP_001276.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chloride channel accessory 1,CACC,CACC1,CLCRG1,CaCC-1,GOB5,hCLCA1,hCaCC-1

Background

This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human CLCA1 protein (active). GTX00147-pro

GTX00147-pro Functional Assay Image

Functional ELISA analysis of GTX00147-pro Human CLCA1 protein (active) which can bind immobilized TMEM16A protein.

Human CLCA1 protein (active). GTX00147-pro

GTX00147-pro Image

WB analysis of GTX00147-pro Human CLCA1 protein (active).

Human CLCA1 protein (active). GTX00147-pro

GTX00147-pro Image

SDS-PAGE analysis of GTX00147-pro Human CLCA1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309