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Human CLCA1 protein (active)

Human CLCA1 protein (active). GTX00147-pro
Human CLCA1 protein (active). GTX00147-pro
Human CLCA1 protein (active). GTX00147-pro

Cat No. GTX00147-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

CLCA1 (Calcium-activated chloride channel regulator 1) is a member of the calcium sensitive chloride conductance protein family. This protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits. It has been reported that CLCA1 activates calcium-dependent chloride channel through the interaction with TMEM16A (anoctamin-1). Besides, there exits similarities between human and mouse TMEM16A in amino acid sequence with the identity of 89.66% . Thus, a functional ELISA assay was conducted to detect the association of recombinant human CLCA1 with recombinant mouse TMEM16A. Briefly, CLCA1 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TMEM16A-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CLCA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of CLCA1 with TMEM16A was in a dose dependent manner.

Observed MW

28 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn416~Arg670 (NP_001276.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chloride channel accessory 1 , CACC , CACC1 , CLCRG1 , CaCC-1 , GOB5 , hCLCA1 , hCaCC-1

Background

This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human CLCA1 protein (active). GTX00147-pro

GTX00147-pro Functional Assay Image

Functional ELISA analysis of GTX00147-pro Human CLCA1 protein (active) which can bind immobilized TMEM16A protein.

Human CLCA1 protein (active). GTX00147-pro

GTX00147-pro Image

WB analysis of GTX00147-pro Human CLCA1 protein (active).

Human CLCA1 protein (active). GTX00147-pro

GTX00147-pro Image

SDS-PAGE analysis of GTX00147-pro Human CLCA1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309