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Human CX3CL1 protein (active)

Human CX3CL1 protein (active). GTX00214-pro
Human CX3CL1 protein (active). GTX00214-pro
Human CX3CL1 protein (active). GTX00214-pro

Cat No. GTX00214-pro

Application

Functional Assay

Species

Human
Package
10 μg ($259)

APPLICATION

Application Note

Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150 μl cell suspension, 1x10⁶ cells/ml in RPMI-1640 with FBS free) and SLC (1 ng/ml, 10 ng/ml, 100 ng/ml and 1000 ng/ml diluted separately in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly. And CX3CL1 is able to induce migration of THP-1 cells.

Observed MW

44 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gln25~Pro332 (NP_002987.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X3-C motif chemokine ligand 1 , ABCD-3 , C3Xkine , CXC3 , CXC3C , NTN , NTT , SCYD1 , fractalkine , neurotactin

Background

This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Human CX3CL1 protein (active). GTX00214-pro

GTX00214-pro Functional Assay Image

The chemotactic effect of GTX00214-pro Human CX3CL1 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1hr.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 10 ng/ml CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1hr.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Human CX3CL1 protein (active). GTX00214-pro

GTX00214-pro Image

WB analysis of GTX00214-pro Human CX3CL1 protein (active).

Human CX3CL1 protein (active). GTX00214-pro

GTX00214-pro Image

SDS-PAGE analysis of GTX00214-pro Human CX3CL1 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 259