APPLICATION
Application Note
Neutrophil-activating protein 3 (NAP3) also known as chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family. NAP3 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of NAP3 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶ cells/ml in RPMI-1640 with FBS free) and SLC (31. 25 ng/ml, 62. 5 ng/ml, 125 ng/ml and 250 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly. And NAP3 is able to induce migration of THP-1 cells.
Observed MW
40 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His and GST-Tag; Ala35~Asn107 (NP_001502.1)
Expression System
E. coli
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
C-X-C motif chemokine ligand 1 , FSP , GRO1 , GROa , MGSA , MGSA-a , NAP-3 , SCYB1
Background
This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]
Database
Research Area
DATA IMAGES
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GTX00215-pro Functional Assay Image
The chemotactic effect of GTX00215-pro Human CXCL1 / GRO alpha protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1hr.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 62.5 ng/ml NAP3 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1hr.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).
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GTX00215-pro Image
WB analysis of GTX00215-pro Human CXCL1 / GRO alpha protein (active).
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GTX00215-pro Image
SDS-PAGE analysis of GTX00215-pro Human CXCL1 / GRO alpha protein (active).
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REFERENCE
There are currently no references for Human CXCL1 / GRO alpha protein, His and GST tag (active) (GTX00215-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human CXCL1 / GRO alpha protein, His and GST tag (active) (GTX00215-pro). Be the first to share your experience with this product.
