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Human CYP1B1 protein, His tag

Human CYP1B1 protein, His tag. GTX00418-pro
Human CYP1B1 protein, His tag. GTX00418-pro
Human CYP1B1 protein, His tag. GTX00418-pro

Cat. No. GTX00418-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($359)
See all CYP1B1 protein products

APPLICATION

Application Note

Cytochrome P450 family 1 subfamily B member 1 (CYP1B1) is an enzyme, Which belongs to the cytochrome P450 superfamily. CYP1B1 can catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. Besides, Heat Shock Protein family B (small) member 2 (HSPb2) has been identified as an interactor of CYP1B1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1B1 and recombinant human HSPb2, CYP1B1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HSPb2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CYP1B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of CYP1B1 and HSPb2 was in a dose dependent manner.

Observed MW

18/16 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp374~Phe516 (NP_000095.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome P450 family 1 subfamily B member 1 , ASGD6 , CP1B , CYPIB1 , GLC3A , P4501B1

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human CYP1B1 protein, His tag. GTX00418-pro

GTX00418-pro Functional Assay Image

Functional ELISA analysis of GTX00418-pro Human CYP1B1 protein which can bind immobilized HSPb2 protein.

Human CYP1B1 protein, His tag. GTX00418-pro

GTX00418-pro Image

SDS-PAGE analysis of GTX00418-pro Human CYP1B1 protein.

Human CYP1B1 protein, His tag. GTX00418-pro

GTX00418-pro Image

WB analysis of GTX00418-pro Human CYP1B1 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 359