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Human Carbonic Anhydrase II protein (active)

Human Carbonic Anhydrase II protein (active). GTX66936-pro

Cat. No. GTX66936-pro

Application

 Functional Assay

Species

 Human
Package
100 μg ($389)
See all Carbonic Anhydrase II protein products

APPLICATION

Application Note

Specific activity is > 7000 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl buffer (pH8.0), 1mM DTT, 50mM NaCl, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, Length: 260 a.a. Sequence: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

carbonic anhydrase 2 , CA-II , CAC , CAII , Car2 , HEL-76 , HEL-S-282

Background

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Human Carbonic Anhydrase II protein (active). GTX66936-pro

GTX66936-pro Image

3 μg of GTX66936-pro Human Carbonic Anhydrase II protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 389