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Human Cardiac Troponin I protein (active)

Human Cardiac Troponin I protein (active). GTX00237-pro
Human Cardiac Troponin I protein (active). GTX00237-pro
Human Cardiac Troponin I protein (active). GTX00237-pro

Cat No. GTX00237-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Troponin I Type 3, Cardiac (TNNI3) is a protein that in humans is encoded by the TNNI3 gene. It is a tissue-specific subtype of troponin I, which in turn is a part of the troponin complex. The TNNI3 gene encoding cardiac troponin I (cTnI) is located at 19q13.4 in the human chromosomal genome. Human cTnI is a 24kDa protein consisting of 210 amino acids with isoelectric point (pI) of 9.87. cTnI is exclusively expressed in adult cardiac muscle. Besides, Troponin T Type 2, Cardiac (TNNT2) has been identified as an interactor of TNNI3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNI3 and recombinant human TNNT2. Briefly, TNNI3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNNT2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNNI3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNNI3 and TNNT2 was in a dose dependent manner.

Observed MW

28 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Met1~Gly203 (NP_000354.4)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

troponin I3, cardiac type,CMD1FF,CMD2A,CMH7,RCM1,TNNC1,cTnI

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human Cardiac Troponin I protein (active). GTX00237-pro

GTX00237-pro Functional Assay Image

Functional ELISA analysis of GTX00237-pro Human Cardiac Troponin I protein (active) which can bind immobilized TNNT2 protein.

Human Cardiac Troponin I protein (active). GTX00237-pro

GTX00237-pro Image

SDS-PAGE analysis of GTX00237-pro Human Cardiac Troponin I protein (active).

Human Cardiac Troponin I protein (active). GTX00237-pro

GTX00237-pro Image

WB analysis of GTX00237-pro Human Cardiac Troponin I protein (active).

REFERENCE
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Package List Price ($)
$ 279